Electrophoresis is the process of exposing dispersed particles to a spatially uniform electric field, causing movement through a medium. In biochemistry, this process separates molecules by size, charge, or binding affinity. When placed in an electric field, an ambiguously charged molecule will travel toward the oppositely charged end of the medium. This clearly displays the charge on the molecule, as a positively molecule would travel toward the negative pool and a negatively charged molecule will travel toward the positive pole. The size of a molecule can be determine by comparing the distance it travels through the medium over a finite time to the distance traveled by a molecule of known size.

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