Applied Biosystems 7500 Fast Real-Time PCR System

The Applied Biosystems 7500 Fast Real-Time PCR System is a real-time PCR system which enables high-speed thermal cycling with run times for quantitative real-time PCR applications of fewer than 40 minutes. The system is an open platform and can be used to analyze samples using custom made or COTs-based PCR assays. The 7500 Fast system is batch-cable and can analyzed a batch of 96 samples per run. It is used for gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +/– assays utilizing internal positive controls.

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Availability: Commercially Available

+1 443 262 9536
2 Radcliff Road
Tewksbury, MA 01876
USA
www.thermofisher.com
Manufacturer
Thermo Fisher Scientific, Inc.
Product Name
Applied Biosystems 7500 Fast Real-Time PCR System
Detection Category
Biological;
Detection Principle
Molecular;
Detection Method
Polymerase Chain Reaction;
Application
Laboratory Analysis;
Product Model Number
4351106
Equipment Type
Instrument
Product Synopsis
The Applied Biosystems 7500 Fast Real-Time PCR System is a real-time PCR system which enables high-speed thermal cycling with run times for quantitative real-time PCR applications of fewer than 40 minutes. The system is an open platform and can be used to analyze samples using custom made or COTs-based PCR assays. The 7500 Fast system is batch-cable and can analyzed a batch of 96 samples per run. It is used for gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +/– assays utilizing internal positive controls.
Availability
Commercially Available
Technology Readiness Level (TRL)
9
tooltip-iconAssociated Scoring Levels4 –TRL 9, immediately available
3 – TRL 9 1-2 months delivery after order
2 – TRL 7-8
1 – <TRL 7
Dimensions
34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D)
Weight
>50 lbs.; 34 kg (75 lb)
tooltip-iconAssociated Scoring Levels4 – <1 lb
3 – <5 Lbs.
2 – <30 Lbs.
1 – >30 Lbs.
Power Requirements
AC/DC Line Power; 120V, 50/60Hz, 8A, 950W
tooltip-iconAssociated Scoring Levels4 – None
3 – Battery or Line Power
2 – Standard Line Power
1 – Non-Standard Line Power
Transportability
Benchtop/Fixed
tooltip-iconAssociated Scoring Levels4 – Handheld
3 – Person-Portable
2 – Mounted
1 – Benchtop/Fixed
Ruggedness
Indoor use only
Operating Conditions
Temperature: 15 to 30 °C (59 to 86 °F). Maximum change of less than 15 degrees Celsius (27 °F) per 24 hrs. Humidity: 20 to 80% relative humidity, noncondensing.
Consumables
>$1,000/year; 96 well plates
tooltip-iconAssociated Scoring Levels4 – No consumables or routine maintenance
3 – Minor consumables (< $1,000/year) and user maintenance
2 – Major consumables (> $1,000/year) and annual vendor provided maintenance
1 – Frequent Service/Manufacturer maintenance required
Calibration Schedule
Monthly/6 months or if moved
Suggested Routine Maintenance
Weekly, monthly and 6 month maintenance
Data Analysis Support Equipment
Computer
Communications Interface
USB
Unit Cost
$35,000.00
tooltip-iconAssociated Scoring Levels4 - <$500
3 – <$10,000 per unit
2 – <$50,000 per unit
1 – >$50,000 per unit
Maintenance Cost
$4,800 annual service contract
Tested For Biological Agents
B.anthracis, F. tularensis, Y. pestis
Other Biological Targets
VEEV, Escherichia coli O157:H7, SARS-CoV-2 (COVID-19), etc. Almost any custom or COTS assays can be analyzed with this instrument
Adaptability
Yes
Sample Introduction
Liquid
Sensitivity/Detection Limits
copies/mL; Down to 1 copy of human RNAse P gene
tooltip-iconAssociated Scoring Levels4 – Detects trace (<µg/ppm)
3 – Detects less than 1% in mixture
2 – Detects < ~10% in mixture
1 – Detects at > 10%
Start Up Time (From Cold Start To Sample Ready)
< 1 minute
Response Time (Sample Application To Output)
>15 minutes; 36 minutes (Fast), 2 hours (Standard)
tooltip-iconAssociated Scoring Levels4 – Continuous/<60 Seconds
3 – <5 minute
2 – <15 minute
1 – > 15 minutes
Total Run Time
36 minutes (Fast), 2 hours (Standard)
Alarm Capability
Visual
Software Control
PC
Training Required
>1 week training
Training Available
Yes; in-person, webinars, videos
Manuals Available
Yes
Warranties
1 year
Data File Type Format
SDS
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