MinION

The MinION is DNA, RNA and protein sensor. The MinION nanopore sensor consists of arrays of nano-scale holes (nanopores) through which sample solution passes. The electrical current across the nanopore is measured. Analytes passing through the pores or near their aperture create a characteristic distribution. Measurement of that current makes it possible to identify the molecule in question. For example, this system can be used to distinguish between the four standard DNA bases G, A, T and C, and also modified bases. It can be used to identify target proteins and small molecules, or to gain rich molecular information, for example to distinguish between the enantiomers of ibuprofen or study molecular binding dynamics.

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Availability: Commercially Available

44 (0)845 034 7900
Edmund Cartwright House, 4 Robert Robinson Avenue
Oxford Science Park, Oxford,OX4 4GA,
UK
https://nanoporetech.com/
Manufacturer
Oxford Nanopore Technologies
Product Name
MinION
Detection Category
Biological
Detection Principle
Molecular;
Detection Method
Single Molecule Real-Time Sequencing;
Application
Laboratory Analysis;
Equipment Type
Instrument
Product Synopsis
The MinION is DNA, RNA and protein sensor. The MinION nanopore sensor consists of arrays of nano-scale holes (nanopores) through which sample solution passes. The electrical current across the nanopore is measured. Analytes passing through the pores or near their aperture create a characteristic distribution. Measurement of that current makes it possible to identify the molecule in question. For example, this system can be used to distinguish between the four standard DNA bases G, A, T and C, and also modified bases. It can be used to identify target proteins and small molecules, or to gain rich molecular information, for example to distinguish between the enantiomers of ibuprofen or study molecular binding dynamics.
Availability
Commercially Available
Technology Readiness Level (TRL)
9
Market Entry Date
May 2015
User Feedback Sources
Indifferent/No user feedback
Supporting Documentation
Docs: Nanopore - MinION.pdf;
Dimensions
4.1 x 0.9 x 1.3 in (10.5 x 2.3 x 3.3 cm)
Weight
<1 lb.; 0.2 lbs (87 g)
Power Requirements
AC/DC Line Power; USB 3 (1 watt)
Transportability
Handheld
Consumables
>$1,000/year; flow cells; sample preparation kits
Solvents/Reagents
Yes
Calibration Schedule
None
Suggested Routine Maintenance
Flow cell replacement
Available Accessories
PromethION larger flow cell
Data Analysis Support Equipment
PC, software
Communications Interface
USB
Expected Operational Life
72 hour flow cell life
Shelf Life
>5 years
Unit Cost
$1,000.00
Maintenance Cost
Flow Cell Cost $270 - $900
Other Biological Targets
DNA, RNA, proteins
Sample Introduction
Liquid
Sensitivity/Detection Limits
pg; µg
Start Up Time (From Cold Start To Sample Ready)
105 minutes
Response Time (Sample Application To Output)
>15 minutes; 1 minute to 48 hours
Total Run Time
1 minute to 48 hours
Software Control
Yes
Other Operational Parameters
Up to 512 channels
Training Required
<1 week training
Manuals Available
Yes
Reachback Service
Yes
Warranties
No
Data File Type Format
.fast5
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