PromethION

The PromethION is a nanopore-based desktop device for high-throughput, high sample-number analysis of biological molecules such as DNA and RNA. The PromethION nanopore sensor consists of arrays of nano-scale holes (nanopores) through which sample solution passes. The electrical current across the nanopore is measured. This system can distinguish between the four standard DNA bases G, A, T and C, and also modified bases. Also to identify target proteins and small molecules, or to distinguish between the enantiomers of ibuprofen.

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Availability: Commercially Available

+1 646 846 4446
New York Genome Center, 101 Avenue of the Americas
New York, NY 10013
East Coast USA
www.nanoporetech.com
Manufacturer
Oxford Nanopore Technologies
Product Name
PromethION
Detection Category
Biological
Detection Principle
Molecular;
Detection Method
Single Molecule Real-Time Sequencing;
Application
Laboratory Analysis;
Product Model Number
P24, P48
Equipment Type
Instrument
Product Synopsis
The PromethION is a nanopore-based desktop device for high-throughput, high sample-number analysis of biological molecules such as DNA and RNA. The PromethION nanopore sensor consists of arrays of nano-scale holes (nanopores) through which sample solution passes. The electrical current across the nanopore is measured. This system can distinguish between the four standard DNA bases G, A, T and C, and also modified bases. Also to identify target proteins and small molecules, or to distinguish between the enantiomers of ibuprofen.
Availability
Commercially Available
Technology Readiness Level (TRL)
9
User Feedback Sources
Indifferent/No user feedback
Supporting Documentation
Docs: Nanopore-promethion.pdf;
Dimensions
17 x 16 x 9 in (59 x 19 x 43 cm)
Weight
>50 lbs.; 60 lbs (28 kg)
Power Requirements
AC/DC Line Power; < 20 watts to 1 kw
Transportability
Benchtop/fixed
Consumables
>$1,000/year; flow cells; sample preparation kits
Solvents/Reagents
Yes
Calibration Schedule
None
Suggested Routine Maintenance
Not Specified
Data Analysis Support Equipment
PC, software
Communications Interface
USB
Expected Operational Life
72 hour flow cell life
Shelf Life
>5 years
Unit Cost
$135,000.00
Maintenance Cost
Flow Cell Cost $270 - $900
Other Biological Targets
DNA, RNA, proteins
Sample Introduction
Liquid
Sensitivity/Detection Limits
pg; µg
Start Up Time (From Cold Start To Sample Ready)
105 minutes
Response Time (Sample Application To Output)
>15 minutes; 1 minute to 48 hours
Total Run Time
1 minute to 48 hours
Other Operational Parameters
Up to 144,000 channels
Training Required
<1 week training
Training Available
Not Specified
Manuals Available
Yes
Reachback Service
Yes
Warranties
No
Data File Type Format
.fast5
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