BioToxin Assays

Acumen Detection’s patent-pending toxin assays deliver fluorescence based, near real-time, one-step detection. The assays also work on PCR platforms, eliminating the need for responders to carry a second instrument to the field to identify toxins.The toxin assays are run at a steady 37° C, and not thermal cycled. The assays are “open architecture”; can be run on virtually any qPCR platform. Positive and negative controls are provided.

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Availability: Commercially Available

Tim Moshier
President
sales@acumendetection.com
+1 315 452 8408
6274 Running Ridge Road
North Syracuse, NY 13212
USA
www.acumendetection.com
Manufacturer
Acumen Detection
Product Name
BioToxin Assays
Detection Category
Biological
Detection Principle
Immunological;
Detection Method
Lateral Flow Hand Held Immunoassay;
Application
Forensics;
Product Model Number
Acu-Bot™, Acu-Ricin™ and Acu-PAPA™ , Acu-Shiga ™
Equipment Type
Instrument
Product Synopsis
Acumen Detection’s patent-pending toxin assays deliver fluorescence based, near real-time, one-step detection. The assays also work on PCR platforms, eliminating the need for responders to carry a second instrument to the field to identify toxins.The toxin assays are run at a steady 37° C, and not thermal cycled. The assays are “open architecture”; can be run on virtually any qPCR platform. Positive and negative controls are provided.
Availability
Commercially Available
Technology Readiness Level (TRL)
8
User Feedback Sources
Department of Defense as part of JBTDS Phase 1, and JUPITR ATD, DHS BioWatch.
Weight
<1 lb.
Power Requirements
None
Transportability
Handheld
Consumables
None
Solvents/Reagents
Assays are lyophilized (freeze dried) in reaction volumes
Expected Operational Life
One time use
Shelf Life
<5 years; 1 to 2 years at 4°C, 1 month to 2 years at 25°C, 10 to 16 weeks at 50°C.
Unit Cost
$14 to $21 per individual assay
Tested For Biological Agents
B. anthracis (Anthrax), F. tularensis (Tularemia), Y. Pestis (Plague), Brucella, C. burnetii (Q fever), pan-orthopox, Venezuelan equine encephalitis (VEE), Ricin, Botulism toxin, Staphlyococcal Enterotoxin B (SEB), Shiga toxin
Sample Introduction
Liquid;
Sensitivity/Detection Limits
ng; Anthrax, Tularemia, Plague, Brucella, Q fever 133 org/mL, SEB 50 ng/mL, Ricin 160 ng/mL, Botulinum 10 ng/mL,
Known Interferents/Inhibitors
Assays are formulated to be tolerant of environmental contaminants (interferents) like dust, humic acid, salts, diesel exhaust, etc. As long as the sample is in an aqueous solution or suspension, our assays can accept it. Extremely heavy inhibitor load in the samples resulting in false negative results.Non-specific proteases in the sample matrix creating false positives in the toxin assays. Negative control assays are provided to be run concurrent with our toxin assays.
False Positive Rates
No false positives were seen in vendor testing.
False Negative Rates
1%
Total Run Time
1 hour
Other Operational Parameters
100 µL sample volume
Training Required
<1 hour training
Training Available
Yes
Manuals Available
Yes
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