Applied Biosystems StepOne and StepOne Plus
The Applied Biosystems™ StepOne™ (48-well) and StepOnePlus™ (96-well) Real-Time PCR
Systems bring low throughput real-time PCR technology to even for first-time users. Both systems measure amplification as it occurs, cycle by cycle, allowing for precise and quantitative measurements during the exponential phase of PCR.
Availability: Commercially Available
| Manufacturer |
| Thermo Fisher Scientific, Inc. |
| Product Name |
| Applied Biosystems StepOne and StepOne Plus |
| Detection Category |
| Biological |
| Detection Principle |
| Molecular; |
| Detection Method |
| Polymerase Chain Reaction; |
| Application |
| Laboratory analysis; |
| Equipment Type |
| Instrument |
| Product Synopsis |
| The Applied Biosystems™ StepOne™ (48-well) and StepOnePlus™ (96-well) Real-Time PCR
Systems bring low throughput real-time PCR technology to even for first-time users. Both systems measure amplification as it occurs, cycle by cycle, allowing for precise and quantitative measurements during the exponential phase of PCR. |
| Availability |
| Commercially Available |
| Technology Readiness Level (TRL) |
| 9 |
| User Feedback Sources |
| Indifferent/No User Feedback |
| Dimensions |
| 9.7 x 19.1 x 20.4 in (24.6 x 48.5 x 51.2 cm) |
| Weight |
| >50 Lbs. |
| Power Requirements |
| AC/DC Line power |
| Transportability |
| Benchtop/Fixed |
| Ruggedness |
| Indoor use only |
| Consumables |
| >$1,000/year |
| Solvents/Reagents |
| Reagents |
| Data Analysis Support Equipment |
| PC, software |
| Communications Interface |
| USB |
| Expected Operational Life |
| Indefinite |
| Shelf Life |
| >5 years |
| Sample Introduction |
| Liquid |
| Sensitivity/Detection Limits |
| copies/mL; 10 copies |
| Response Time (Sample Application To Output) |
| >15 minutes |
| Total Run Time |
| 40 minutes |
| Alarm Capability |
| None |
| Software Control |
| On-board; PC; Remote |
| Other Operational Parameters |
| Linear dynamic range: 9 logs. Both StepOne systems are equipped with fluorophore detection chemistries that include FAM and VIC dye– labeled Applied Biosystems™ TaqMan™ MGB probe-based assays, VIC and TAMRA dye–labeled probe-based assays,
and SYBR Green I dye chemistry. |
| Training Required |
| >1 week training |
| Training Available |
| In-person or virtual |
| Manuals Available |
| Yes |
| Warranties |
| Yes |
| ITAR/EAR Export Regulations |
| None |
| IATA Shipping Restrictions |
| None |
