Nano–Intelligent Detection System (NIDS®)
Nano–Intelligent Detection System (NIDS®) BioThreat Rapid Assay and Handheld Reader. This platform utilizes Lateral Flow Immunochromatography (LFI) in which capture antibodies are immobilized onto the substrate surface. Liquid sample is introduced onto the card and a wicking process forces a diffusion of the liquid analyte along a path toward interaction between the capture antibody and the analyte. A colorimetric change takes place if the analyte is present. ANP markets a multianalyte LFI strip for screening against several pathogens/toxins assembled in a microarray format. LFI strips are analyzed by inserting into a reader that is colorimetric based. Single target assays and multiplexes in 2-plex, 3-plex, 4-plex, and 5-plex formats are available.
Availability: Commercially available
| Manufacturer |
| ANP Technologies, Inc. |
| Product Name |
| Nano–Intelligent Detection System (NIDS®) |
| Detection Category |
| Biological |
| Detection Principle |
| Immunological; |
| Detection Method |
| Lateral Flow Hand Held Immunoassay; |
| Application |
| Detection;Emergency Response; |
| Product Model Number |
| SARV |
| Equipment Type |
| Instrument |
| Product Synopsis |
| Nano–Intelligent Detection System (NIDS®) BioThreat Rapid Assay and Handheld Reader. This platform utilizes Lateral Flow Immunochromatography (LFI) in which capture antibodies are immobilized onto the substrate surface. Liquid sample is introduced onto the card and a wicking process forces a diffusion of the liquid analyte along a path toward interaction between the capture antibody and the analyte. A colorimetric change takes place if the analyte is present. ANP markets a multianalyte LFI strip for screening against several pathogens/toxins assembled in a microarray format. LFI strips are analyzed by inserting into a reader that is colorimetric based. Single target assays and multiplexes in 2-plex, 3-plex, 4-plex, and 5-plex formats are available. |
| Availability |
| Commercially available |
| Technology Readiness Level (TRL) |
| 9 |
| Market Entry Date |
| 2008 |
| User Feedback Sources |
| DoD |
| Supporting Documentation |
| Docs: ANP - NIDS.pdf;
Images: NIDS BioThreas-061.jpg; |
| Dimensions |
| 4.6 x 2.3 x 3.7 in (11.7 x 5.7 x 9.4 cm) |
| Weight |
| <30 lbs.; 0.73lbs (330g) reader, 10 lbs (4.5 kg) complete kit |
| Power Requirements |
| AC/DC Line Power; Alkaline Battery; 5V DC power adapter equipped with type A ‘American’ two-prong plug
Rated for input of 100-240VAC, 50/60Hz, 150mA, 3 AA batteries (6 to 7 hours) |
| Transportability |
| Handheld |
| Suggested Routine Maintenance |
| Removable base for easy cleaning and decontamination after a positive result. |
| Available Accessories |
| Comes with 10 assay pairs per system |
| Data Analysis Support Equipment |
| PC, NIDS SARIV PC Software & Microsoft Excel |
| Communications Interface |
| Mini USB port |
| Shelf Life |
| <1 year |
| Unit Cost |
| $13,200 - $20,000 |
| Maintenance Cost |
| $20 - $125 sample cost |
| Tested For Biological Agents |
| Protein antigens—either whole cells or protein toxins including B. anthracis (anthrax), S. aureus (SEB), Ricin, F. tularensis (tularemia), Brucella, C. burnetii (Q fever), Botulinum toxin A, Y. pestis (plague), V. cholera (cholera) |
| Other Biological Targets |
| Vaccinia, Venezuelan Equine Encephalitis, Salmonella, Listeria, E.coli 0157 |
| Adaptability |
| Yes; user can add new targets to detector with support |
| Sample Introduction |
| Liquid |
| Sensitivity/Detection Limits |
| ng; cfu/mL; pfu/mL; LOD for each test assay: Bacillus anthracis—1000 CFU/mL; Botulinum toxin—25 ng/mL; Brucella melitensis—106 CFU/mL; Orthopoxvirus—1E5 PFU/mL; Ricin toxin—12 ng/mL; Yersinia pestis—103 CFU/mL; SEB - 4.5ng/mL. Multiplexing capability using a protein microarray for proteomic applications. |
| Known Interferents/Inhibitors |
| NIDS-3000CA tested against comprehensive panel of interfering substances containing 26 substances from both an Interference Material Reference Panel and 16 substances from a Suspicious Powders Reference Material Panel. Dipel caused a slight false positive on the F. tularensis test line. |
| False Negative Rates |
| Virtually none due to elimination of the Hook Effect |
| Start Up Time (From Cold Start To Sample Ready) |
| 15 Seconds |
| Response Time (Sample Application To Output) |
| >15 minutes |
| Total Run Time |
| 15 minutes |
| Alarm Capability |
| Yes |
| Software Control |
| PC software provided with the system allows users to manage data, update firmware, and change settings using the NIDS standalone reader PC software. |
| Other Operational Parameters |
| Lateral Flow Immunochromatography |
| Training Required |
| <1 day training |
| Training Available |
| Yes |
| Manuals Available |
| Yes |
| Reachback Service |
| Yes |
| Warranties |
| 1 year from date of sale |
| Approved Vendors |
| Federal Resources |
