Home > Chemical > Technology > Molecular Spectroscopy > Ultraviolet-Visible (UV-Vis)

Ultraviolet-visible (UV-Vis) spectroscopy is a technique that identifies chemicals based on the interaction of molecules with electromagnetic radiation in the ultraviolet-visible region (10,000-33,333cm-1 or 300-1000 nm). Molecular absorbances of UV-Vis light cause electronic transitions within molecules. These transitions typically occur with the presence of a transition metal ion, or a conjugated organic molecule. There are UV-Vis reference databases, but there is much less specificity in a UV-Vis spectrum compared to MIR data, making it a secondary verification technique with unknown chemicals. Point analysis (“bench top”) systems are used for analyzing liquid phase materials, although a few systems are also capable of analyzing gases and solids. UV-Vis instrumentation has been scaled down to hand-held size for specific colorimetric applications, but most research-grade instruments are of larger size, and are often combined with NIR instrumentation. Instrument are available that have either single light paths or multiple beams for simultaneous analysis of reference samples.   Additionally, some instruments are capable of making time-resolved measurements for kinetics experiments.    UV-Vis is an extremely mature analytical technique.  Many instruments are available commercially.  Cost widely variable and depends upon both the instrument configuration and the sophistication of the data analysis software.

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600 Series UV Fluorescent SO2

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Focus LC

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